Written by: Noelle A. Koo (12th grade / Ashburn, VA USA)
Another major technique used in disease and viral agent detection is the expression of recombinant proteins. In this research paper, the expression of recombinant protein for the detection of the influenza virus antibody was outlined. Recombinant proteins are "proteins encoded by recombinant DNA that has been cloned in an expression vector that supports expression of gene and translation of messenger RNA" (according to Enzo Life Sciences). Recombinant proteins have been utilized in many studies focusing on therapeutic effects of protein therapies and treatments on various diseases. In this paper the use of recombinant protein technology for the detection of influenza virus antibody was analyzed in which the NSI gene was purified and cloned into a vector and transformed into cells to get recombinant plasmids which were then transformed into the host cell strain B12 pLysS for expression. Analysis of the protein expression through SDS-PAGE and later confirmed by western-blotting showed that the antigen could be utilized to detect specific antibodies against influenza viruses using recombinant protein technology (ELISA - enzyme-linked immunoassay).
In conclusion, the study established that cell culture and technology were necessary factors in modern day medicine and its applications in the realm of disease and infection diagnosis are multifaceted. It was ultimately recommended that cell culture be used with the techniques outlined in the study and for establishing "a rapid test for newly discovered pathogens". This research paper provided insight into the roles of cell culture technology in the progression of modern day research.
Research Paper citation:
Hudu, S. A., Alshrari, A. S., Syahida, A., & Sekawi, Z. (2016). Cell Culture, Technology: Enhancing the Culture of Diagnosing Human Diseases. Journal of clinical and diagnostic research : JCDR, 10(3), DE01–DE5. https://doi.org/10.7860/JCDR/2016/15837.7460
Link to paper: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4843260/
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